Optimized CUBIC protocol for 3D imaging of chicken embryos at single-cell resolution
نویسندگان
چکیده
The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell-level resolution in whole earlystage chicken embryos, and in the whole brains of late-stage embryos. D ev el o pm en t • A dv an ce a rt ic le
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تاریخ انتشار 2017